Journal: Channels

Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice

doi: 10.1080/19336950.2025.2611704

Figure Lengend Snippet: Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.

Article Snippet: Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA).

Techniques: Expressing, Immunofluorescence, Membrane, Western Blot

Journal: Antioxidants (Basel, Switzerland)

Article Title: Structural Optimization of Cannabidiol as Multifunctional Cosmetic Raw Materials.

doi: 10.3390/antiox12020314

Figure Lengend Snippet: Figure 7. Effects of CBD, S-88745 and S-92153 on the expression of iNOS and COX-2 induced by LPS. In addition to an untreated control group and the LPS−treated group, RAW264.7 cells pretreated with CBD, S-88745 and S-92153 at 0.04, 0.4, and 4 µM for 1.5 h, and then treated all groups with LPS, except the control group for 20 h. The levels of iNOS and COX-2 in the culture supernatants of RAW264.7 macrophages were then detected by western blot. (A) iNOS and COX-2 protein expression levels. (B) Relative ration analysis of iNOS expression. (C) Relative ration analysis of COX-2 expression. The experiments were repeated three times. Each bar illustrates the average ± standard deviation (SD) counted from three experiments. p < 0.05 was considered statistically significant. * p < 0.05 compared to the LPS−treated group; ** p < 0.01 compared to the LPS−treated group; *** p < 0.001 compared to the LPS−treated group; **** p < 0.0001 compared to the LPS−treated group; # p < 0.0001 compared to the control group.

Article Snippet: IL‐6 kits were bought from Elab‐ science (Elabscience, Wuhan, China). iNOS antibody and COX‐2 antibody were pur‐ chased from Signalway Antibody (Signalway Antibody, College Park, MA, USA) CBD and the hit compounds were purchased from TargetMol (TargetMol, Shanghai, China).

Techniques: Expressing, Control, Western Blot, Standard Deviation

Journal: Theranostics

Article Title: Acellular cardiac scaffolds enriched with MSC-derived extracellular vesicles limit ventricular remodelling and exert local and systemic immunomodulation in a myocardial infarction porcine model

doi: 10.7150/thno.72289

Figure Lengend Snippet: Scaffold implantation modulates the systemic immune response post-MI, and cATMSC-EV promote a local effect. ( A-C ) Systemic analysis of the immune response post-MI. ( A ) The increase in the number of total peripheral blood mononuclear cells (PBMC) (left) and specifically in the number of lymphocytes (right) in whole blood that occurs 2 days after MI (red) is prevented by scaffold implantation. ( B ) The increase in the number of CCR2 + monocytes observed 30 days post-MI in untreated animals is blocked by scaffold implantation. ( C ) EV-Treated animals reduce the expression of CD73 in circulating monocytes 30 days from MI. Bars indicate mean ± SD and each data point corresponds to one animal (n = 4;7;8 respectively). Statistical significance was calculated with a paired Two-way ANOVA with Tukey's posthoc analysis. ( D-E ) Representative images ( D ) and quantification ( E ) of the number of infiltrating macrophages (CD163 + ; left), of which expressed CD73 (middle), and cells expressing CD73 (right) by immunohistofluorescence analysis at 30 days post-MI. Statistical significance was calculated with a One-way ANOVA with Tukey's posthoc analysis (Infarct core, left) and Student T-test (Scaffold, right). ( E ) CD163 (red), CD73 (green) and DAPI (blue).

Article Snippet: On one hand, 100 μL of whole blood were stained with the primary antibodies mouse anti-pig CD14-FITC (BioRad), anti-pig CD16-PE (BioRad), anti-human CCR2 (CD192)-PE-Vio770 (Miltenyi Biotech), rabbit anti-pig CD73 (Novus Biologicals) and mouse anti-pig CD163 (Novus Biologicals).

Techniques: Expressing, Immunohistofluorescence